Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Article Title: A Systematic Characterization of Aquaporin-9 Expression in Human Normal and Pathological Tissues.
doi: 10.1369/0022155416641028
Figure Lengend Snippet: Figure 1. Protein expression of AQP9 in normal tissues. (A) High membranous expression was observed in hepatocytes. (B) Medium level cytoplasmic expression was found in a subset of lymphoid cells in appendix (a, b). In bone marrow (C) and cells in red pulp of spleen (D), a subset of cells showed medium level cytoplasmic expression (c-f). (E) Moderate AQP9 immunoreactivity was also observed in cytoplasm of a subset of non-germinal center cells in tonsil (g, h). (F) In kidney, endothelial cells of renal glomeruli (i) displayed medium level expression, whereas renal tubules (j) showed weak expression. Scale, 100 µm.
Article Snippet: Anti-AQP9 antibodies sc-28623 and sc-74409 (Santa Cruz Biotechnology), as well as two internally generated antibodies were also tested on the immunohistochemistry platform but were evaluated as not suitable due to nonspecific staining and inconsistency with RNA-seq data (Supplementary Fig. 1).
Techniques: Expressing